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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has actually been noticed in lots of tumors, which results in chromosome instability, and thus may add to tumorigenesis. This protein was observed to associate with, and become linked to, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and performs a vital role in promoting mobile-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, are already described. Two supplemental splice variants have been reported but in-depth nucleotide sequence data just isn't yet offered.
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Blocking peptides are peptides that bind precisely to your target antibody and block antibody binding. These peptide typically incorporates the epitope acknowledged because of the antibody.
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This tutorial outlines even more achievable optimization of cellular and immunoassay parameters to ensure the very best benefits are...
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This manual outlines additional achievable optimization of cellular and immunoassay parameters to be sure the absolute best benefits are...
Antibodies certain to the blocking peptide no more bind on the epitope about the focus on protein. By comparing the staining from your blocked antibody compared to the antibody alone, one can see which staining is unique.
Concentrate on Information and facts Cyclin E belongs to your remarkably conserved cyclin relatives, whose customers are characterized by a dramatic periodicity in protein abundance in the mobile cycle. Cyclins purpose as regulators of CDK kinases. Diverse cyclins exhibit distinctive expression and degradation designs which contribute to the temporal coordination of each and every mitotic function. Cyclin E types a posh with and features like a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein here accumulates with the G1-S stage boundary and is particularly degraded as cells development via S section.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by each antibodies...
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